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Comparison of two multiplex (biochip) methods for determination of specific IgE against molecular defined allergens
Velčovská, Denisa ; Andrýs, Ctirad (advisor) ; Jankovičová, Karolína (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Subject of study: Bioanalytical laboratory diagnostics in health care Author: Bc. Denisa Velčovská Supervisor: prof. RNDr. Ctirad Andrýs, Ph.D. Title of diploma thesis: Comparison of two multiplex (biochip) methods for determination of specific IgE antibodies against food allergens in group of patients suffering from atopic dermatitis This thesis deals with the comparison of two multiplex (biochip) methods ALEX and ISAC in the determination of specific IgE antibodies against selected allergens in patients with atopic dermatitis. The aim of this thesis is to statistically compare the two methods and perform an evaluation of the data obtained, to assess whether the two methods provide similar results and are interchangeable, or whether a statistically significant difference can be observed. This diploma thesis mainly focuses on inhalant allergens of moulds, mites, house dust mites and animal allergens. The theoretical part of this diploma thesis is focused on allergies and their clinical forms. It also describes allergens, their nomenclature, biochemical structure and methods used to diagnose allergic inflammation. In the experimental part, the basic statistics of the cohort, which includes 96...
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Allergic phenotypes in patients with respiratory allergy evaluated by microchip ISAC technique
Porubová, Petra ; Andrýs, Ctirad (advisor) ; Drahošová, Marcela (referee)
Charles University Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Author: Bc. Petra Porubová Supervisor: prof. RNDr. Ctirad Andrýs, Ph.D. Title of diploma thesis: Allergic phenotypes in patients with respiratory allergy evaluated by microchip ISAC technique Key words: allergic inflammation, allergen specific IgE, component resolved diagnostics, microchip, ISAC Background: The aim of this diploma thesis was to evaluate the sensitization profile in the group of pacients with allergic rhinitis and/or bronhial asthma and to identify possible differences between patients with different disease severity. Methods: ImmunoCAP ISAC was used to determine specific IgE antibodies against 112 allergen components. The evaluated group included 152 patients (57.9 % women) from the allergology outpatient office of the Department of Clinical Immunology and Allergology, University Hospital Hradec Králové. Results: The most common allergens in this group of patients were the components Phl p 1 (68,4 %) and Bet v 1 (65,1 %). Differences in the number of positive and negative reactions were demonstrated between patients with different severity of allergic rhinitis. Correlations were found between the number of positive/negative reactions and the severity of bronchial asthma. And the...
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